powerquant® kit  (Promega)

Journal: Genes

Article Title: Molecular Identification of the Italian Soldiers Found in the Second World War Mass Grave of Ossero

doi: 10.3390/genes16030326

Figure Lengend Snippet: ( a ) Analyses performed by Quantifiler. ( b ) Analyses performed by PowerQuant.

Article Snippet: The remaining 72 samples were quantified using the PowerQuant TM System kit (Promega, Madison, WI, USA) with the 7500 Real-Time PCR System for Human Identification, HID Real-time PCR analysis (Applied Biosystem, AB, Foster City, CA, USA).

Techniques:

Journal: BMC Medical Genomics

Article Title: Exploring the potential of genetic analysis in historical blood spots for patients with iodine-deficient goiter and thyroid carcinomas in Switzerland and Germany (1929–1989)

doi: 10.1186/s12920-024-01947-y

Figure Lengend Snippet: Overview of the PowerQuant primers and probes for DNA quantification using the PowerQuant ® System. Data from the four dye channels are normalized to the passive reference dye, CXR dye (adopted from [ 37 ])

Article Snippet: The quantification of DNA was carried out in triplicates using the Powerquant ® kit (Promega Corporation, Madison, WI, USA) on an Applied BiosystemsTM 7500 Real Time PCR System (Thermo Fisher Scientific, Waltham, MA, USA) following the manufacturers’ protocols [ , ], except for the internally validated reaction in half volume.

Techniques: Amplification, Labeling, Control

Journal: BMC Medical Genomics

Article Title: Exploring the potential of genetic analysis in historical blood spots for patients with iodine-deficient goiter and thyroid carcinomas in Switzerland and Germany (1929–1989)

doi: 10.1186/s12920-024-01947-y

Figure Lengend Snippet: Interpretation guidance for degradation or inhibition, potentially occurring in quantified samples using the PowerQuant ® System. The thresholds for degradation or inhibition were adapted according to internal validation studies with a threshold value of 10 for [Auto]/[Deg] and 5 for the IPC shift. “No Deg Cq” = no degradation quantification cycle [ 37 ]

Article Snippet: The quantification of DNA was carried out in triplicates using the Powerquant ® kit (Promega Corporation, Madison, WI, USA) on an Applied BiosystemsTM 7500 Real Time PCR System (Thermo Fisher Scientific, Waltham, MA, USA) following the manufacturers’ protocols [ , ], except for the internally validated reaction in half volume.

Techniques: Inhibition, Biomarker Discovery